Table 6.

Effect of dietary supplementation of Vit E/Se on relative mRNA abundance of oxidative stress and immunity related genes in the liver of heat-stressed broiler chickens on d 35.¹

	Treatments2				Diet		Ambient Temperature			Statistics3
	Control		Vit E/Se							P-value
Item	TN	HS	TN	HS	Control	Vit E/Se	TN	HS	RMSE	Diet	AT	D × AT
HSP70	1.14c	13.96a	1.02c	4.97b	7.55	3.00	1.08	9.47	4.49	0.001	<0.001	0.001
HSP90	1.24c	5.97a	1.25c	3.65b	3.60	2.45	1.25	4.81	1.74	0.015	<0.001	0.014
SOD	1.06	1.19	1.13	1.06	1.13	1.10	1.10	1.13	0.37	0.755	0.750	0.299
GPx	1.13	2.31	1.16	2.39	1.72	1.77	1.14	2.35	1.26	0.866	<0.001	0.941
mTOR	1.09	0.85	0.82	0.70	0.97	0.76	0.96	0.78	0.35	0.018	0.046	0.515
TLR2	1.20	1.00	0.85	0.60	1.10	0.73	1.02	0.80	0.53	0.006	0.096	0.851
TLR4	1.22	1.00	0.84	0.77	1.11	0.81	1.03	0.88	0.65	0.070	0.370	0.641
IL-1β	1.09	0.37	0.82	0.34	0.73	0.58	0.96	0.36	0.29	0.047	<0.001	0.101
IL-4	1.05	0.88	0.95	0.99	0.96	0.97	1.00	0.93	0.46	0.944	0.553	0.352
IL-10	1.21	1.46	0.70	0.68	1.33	0.69	0.95	1.07	0.78	0.002	0.552	0.497
TNFα	1.11	0.93	0.82	0.61	1.02	0.72	0.97	0.77	0.52	0.025	0.141	0.935
iNOS	1.06	1.02	0.65	0.98	1.04	0.82	0.85	1.00	0.43	0.050	0.185	0.092
IFNγ	1.26	0.85	0.74	0.40	1.05	0.57	1.00	0.62	0.56	0.001	0.009	0.778

^a-cMeans with different superscripts in the same row are significantly different (P < 0.05).

¹Data represent mean values of 16 replicates per treatment.

²Control: birds fed a basal diet; Vit E/Se: birds fed a basal diet supplemented with 250 mg/kg vitamin E and 1 mg/kg selenium; TN: thermoneutral; HS: heat stress

³AT: Ambient Temperature; D: Diet.