Fig. 5.

Penetrance microscopy assays. A-B. Confocal 3D image of MSC and dVSMC at day 14 in the patch. In red is represented vimentin, cell nuclei are stained in blue with DAPI. In red is represented vimentin, cell nuclei are stained in blue with DAPI. C-J. Scanning electron microscopy of both sides of the patch at day 14. C–F. Upper surface: Low nanofiber density and larger gaps. This is the graft side where cells are seeded, deposit and gravity-fed through the pores of the patch. G-J. Bottom surface: This is the lower side of the graft where cells cannot be strained due to high density of nanofiber and smaller gaps.