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. 2022 Apr 16;14:100262. doi: 10.1016/j.mtbio.2022.100262

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© 2022 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 3 — Cytocompatibility, proliferation and adhesion of endothelial cells on coated ePTFE grafts. An increased number of viable cells was observed on hpcECM-gel coated grafts (Calcein AM/Propidium Iodide staining) after 24 h (A), but no significant changes in cell viability (B) or proliferation rates (C) were observed over a time period up to 96 h. HUVEC adhesion was significantly upregulated in cells seeded on hpcECM-gel compared to fibronectin or non-coated surfaces after 10 min of seeding time (D). Proliferation rates of cells which showed higher attachment in the adhesion assay however do not proliferate faster on hpcECM-gel compared to the other conditions (E). An increased number of EPCs were found on hpcECM-gel coated ePTFE grafts after one week of incubation (Prom-1 staining) (F). Scale bars = 50 μm, n = 6.