Figure 6.

Adoptive transfer of TCRαβ⁺DNT cells prevented the development and progression of NAFLD. (A) Serum ALT and serum AST levels were measured in NCD- and MCD-fed mice and TCRαβ⁺ DNT cell-transferred mice. (B) Representative H&E staining and Oil Red O and α-SMA staining in livers from NCD- and MCD-fed mice and TCRαβ⁺ DNT-transferred mice. (C) NAFLD activity score (NAS) in livers of NCD- and MCD-fed mice and TCRαβ⁺ DNT-transferred mice. (D–F) Representative flow cytometry plots and statistical analysis of proportion of CD4⁺ and CD8⁺ Treg cells among livers of NCD- and MCD-fed mice and TCRαβ⁺DNT cell-transferred mice. (G) Representative flow cytometry plots and statistical analysis of apoptosis of intrahepatic CD4⁺ and CD8⁺ cells of NCD- and MCD-fed mice and TCRαβ⁺DNT cell-transferred mice. n ≥ 4 mice/group. Two-sided P values <.05 were considered significant. ∗∗P < .01; ∗P < .05.