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. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Cancer Lett. 2022 Mar 20;535:215659. doi: 10.1016/j.canlet.2022.215659

Figure 2. Prkaa1 deleted cells have no AMPK activity.

Figure 2.

A) Western blot for Prkaa1 (α1-AMPK) from the parental line (MFL2), control Rosa26 targeted control cells or Prkaa1 deleted cells (AMPK KO). Actin served as a loading control. B) Western blot for Prkaa1 and Prkaa2 (T-αAMPK). Actin served as a loading control. C) Western blot for phosphorylated ACC1 (p-ACC1) and total ACC1 (t-ACC1) in Hank’s balanced salt solution (HBSS) with and without compound C (Comp.C). Actin served as a loading control. D) Viability assays. Rosa26 control or AMPK KO cells were treated with the indicated amount of the glycolysis inhibitor 2-deoxyglucose (2DG) for 72 hours and viability assessed. *=p value <0.05, ****=p value <0.001.