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Tris-buffered saline (TBS)
| Reagent | Final concentration | Amount |
|---|---|---|
| Tris hydrochloric acid | 0.436% | 17.44 g |
| Tris(hydroxymethyl)aminomethane | 0.064% | 2.56 g |
| Sodium chloride | 0.8% | 32 g |
| Potassium chloride | 0.02% | 0.8 g |
| dH2O | n/a | 4 L |
| Total | n/a | 4 L |
Prepared TBS can be stored at room temperature (approximately 20°C–22°C) for up to 1 week or 4°C for up to 2 weeks.
