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. 2022 Apr 14;11(2):e1283. doi: 10.1002/mbo3.1283

Table A3.

Media used for bacterial cultures

Media recipes
LB broth (1 L) 10 g Tryptone (pancreatic digest of casein)
5 g Yeast extract
5 g NaCl
(+15 g agar for solid media)
CPG broth (1 L) 1 g Casamino acids (casein hydrolysate)
10 g Peptone
5 g Glucose
(+17 g agar for solid media)
NB broth (1 L) 10 g Glucose
5 g Tryptone
3 g Beef extract
0.5 g Yeast extract
Kings Medium B broth (1 L) 20 g Proteose peptone
1.5 g K2HPO4
1.5 g MgSO4·7H2O
10 ml Glycerol
Cryomedia for freezing bacterial stocks 50% vol/vol glycerol