Table A3.
Media recipes | |
---|---|
LB broth (1 L) | 10 g Tryptone (pancreatic digest of casein) |
5 g Yeast extract | |
5 g NaCl | |
(+15 g agar for solid media) | |
CPG broth (1 L) | 1 g Casamino acids (casein hydrolysate) |
10 g Peptone | |
5 g Glucose | |
(+17 g agar for solid media) | |
NB broth (1 L) | 10 g Glucose |
5 g Tryptone | |
3 g Beef extract | |
0.5 g Yeast extract | |
Kings Medium B broth (1 L) | 20 g Proteose peptone |
1.5 g K2HPO4 | |
1.5 g MgSO4·7H2O | |
10 ml Glycerol | |
Cryomedia for freezing bacterial stocks | 50% vol/vol glycerol |