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. Author manuscript; available in PMC: 2022 May 2.
Published in final edited form as: Cell Rep. 2020 Feb 11;30(6):1898–1909.e4. doi: 10.1016/j.celrep.2020.01.022

Figure 2. Mitochondrial OXPHOS Controls the Fate Decision between Pathogenic Th17 and Treg Cell Development.

Figure 2.

(A) 2D2 CD4 T cells were differentiated under Th17-polarizing conditions in the presence of vehicle or oligomycin for 5 days and transferred into Rag1−/− recipients. Disease symptoms were monitored daily (n = 9; 2 independent experiments).

(B) Heatmap illustrates relative expression of RNA sequencing data of genes associated with Th17 cells.

(C and D) Naive CD4 T cells were activated under Th17 conditions in the presence of vehicle or oligomycin for 72 h. Representative plots are gated on live CD4 T cells (C) and graphs (D) show the expression of IL-17A, Foxp3, and RORγt (representative of 12 independent experiments).

(E) Foxp3+ (GFP+) CD4 T cells were isolated after Th17 differentiation in the presence of oligomycin and co-cultured with proliferation dye labeled CD45.1+ WT naive CD4 T cells at a 1:1 ratio. Representative plots show proliferation of CD45.1+ WT CD4 T cells on day 3 (3 independent experiments).

Graphs show the average ± SEM (A) or SD (D and E); (A and E) two-way ANOVA; (D) unpaired t test *p < 0.05, **p < 0.01, ***p < 0.001.