Figure 5. mTORC1 Activation during Th17 Differentiation Requires OXPHOS and Supports BATF Induction.
(A) Naive CD4 T cells from Nur77/GFP mice were activated under Th17 conditions in the presence of vehicle or oligomycin for 1 h. Phosphorylation of S6 is measured by flow cytometry (representative of 3 independent experiments).
(B) Relative expression of indicated genes derived from RNA sequencing is shown (vehicle Th17 versus oligomycin Th17). (C–F) Naive CD4 T cells were activated under Th17 conditions in the presence of vehicle or the indicated inhibitor for 24 h. Phosphorylation of (C) S6 and (D) 4E-BP1 were analyzed.
(E and F) BATF expression was analyzed in (E and F) vehicle- or (F) inhibitor-treated cells (6–9 independent experiments).
Graphs show the average ± SD; (A, C, D, and E) unpaired t test; (F) one-way ANOVA *p < 0.05, **p < 0.01, ***p < 0.001.