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. Author manuscript; available in PMC: 2022 May 2.
Published in final edited form as: Cell Rep. 2020 Feb 11;30(6):1898–1909.e4. doi: 10.1016/j.celrep.2020.01.022

Figure 5. mTORC1 Activation during Th17 Differentiation Requires OXPHOS and Supports BATF Induction.

Figure 5.

(A) Naive CD4 T cells from Nur77/GFP mice were activated under Th17 conditions in the presence of vehicle or oligomycin for 1 h. Phosphorylation of S6 is measured by flow cytometry (representative of 3 independent experiments).

(B) Relative expression of indicated genes derived from RNA sequencing is shown (vehicle Th17 versus oligomycin Th17). (C–F) Naive CD4 T cells were activated under Th17 conditions in the presence of vehicle or the indicated inhibitor for 24 h. Phosphorylation of (C) S6 and (D) 4E-BP1 were analyzed.

(E and F) BATF expression was analyzed in (E and F) vehicle- or (F) inhibitor-treated cells (6–9 independent experiments).

Graphs show the average ± SD; (A, C, D, and E) unpaired t test; (F) one-way ANOVA *p < 0.05, **p < 0.01, ***p < 0.001.

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