Gastric cancer serum aptamers SELEX screening conditions.
Round | MBs (mL) | ssDNA library (pmol) | ssDNA library into each round (pmol) | Gastric cancer serum (μL) | Library/MBs-serum | Incubation time (min) |
---|---|---|---|---|---|---|
1 | 4 | 69.5 | 69.5 | 600 | 2 | 60 |
2 | 4 | 46.3 | 46.3 | 300 | 2 | 60 |
3 | 3 | 79.8 | 52 | 350 | 4 | 50 |
4 | 3 | 59.7 | 50 | 300 | 4 | 50 |
5 | 2 | 42.9 | 40 | 275 | 6 | 50 |
6 | 2 | 51.6 | 45 | 250 | 6 | 40 |
7 | 2 | 49.1 | 40 | 225 | 8 | 40 |
8 | 1.5 | 30.1 | 30 | 200 | 8 | 40 |
9 | 1 | 28.1 | 28 | 175 | 10 | 30 |
10 | 1 | 31.0 | 29.6 | 150 | 10 | 30 |