Effect of subtilisin A on the Oxysept 1 Step hydrogen peroxide contact lens disinfection system against A. polyphaga cysts. The last time point shown indicates the total kill of the challenge inoculum. ○, subtilisin A (Ultrazyme); ▵, Oxysept 1 Step; □, Oxysept 1 Step + catalase + subtilisin A; ◊, Oxysept 1 Step + catalase; ●, Oxysept 1 Step + subtilisin A.