Fig. 6. Western blot verification of marker gene expression. The protein expression levels of COL-I, ALP, OCN, BMP-2, RUNX-2 were measured with a western blot. The results showed the same trend as the mRNA levels. TNF-α down-regulated the protein expression of the six genes and it returned to a relatively high level (p < 0.05) with the presence of PLX-4720.
