Skip to main content
. Author manuscript; available in PMC: 2022 May 2.
Published in final edited form as: Nat Protoc. 2022 Feb 23;17(4):1004–1027. doi: 10.1038/s41596-021-00669-0

Fig. 1 ∣. The steps of lentivirus packaging and transduction.

Fig. 1 ∣

The protocol has seven parts: (1) preparation of lentivirus vector with the CRISPR–Cas9 targeting gene of interest; (2) lentivirus packaging; (3) lentivirus transduction of HIEs; (4) HIE recovery and antibiotic selection; (5) single-cell cloning; (6) sequencing verification of single-cell clones; (7) using KO lines to evaluate host gene function in viral replication or other biological processes.