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. Author manuscript; available in PMC: 2022 May 2.
Published in final edited form as: Nat Protoc. 2022 Feb 23;17(4):1004–1027. doi: 10.1038/s41596-021-00669-0

Fig. 3 ∣. HIE morphologies during transduction and puromycin selection.

Fig. 3 ∣

a, Single-cell HIEs for lentiviral transduction at day 0. b, Expanded single-cell clones after 2 d of transduction. c,d, Expanded HIE clones 4 d after transduction, which are ready to be selected with puromycin. e, Transduced HIEs treated with puromycin for 4 d. f, Nontransduced HIEs treated with puromycin for 4 d with dying and dead cells. 20× objective lens.