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. Author manuscript; available in PMC: 2022 May 2.
Published in final edited form as: Nat Protoc. 2022 Feb 23;17(4):1004–1027. doi: 10.1038/s41596-021-00669-0

Fig. 4 ∣. The selection criteria for single-cell HIE clones.

Fig. 4 ∣

Different HIE clones can be observed in each well of 96-well plates under a bright-field microscope. a,b, Surviving single-cell clones growing from individual transduced HIEs. c, A surviving and expanding single-cell clone that is ready for passaging. d-f, Dying or dead single-cell clones that need to be excluded. g,h, Multiple surviving clones in one well that need to be excluded as well since they are mixed populations. 20× objective lens.