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. Author manuscript; available in PMC: 2022 May 2.
Published in final edited form as: Nat Protoc. 2022 Feb 23;17(4):1004–1027. doi: 10.1038/s41596-021-00669-0

Fig. 5 ∣. The comparison between KO and wild-type sequencing results.

Fig. 5 ∣

a, According to the sequencing result of the genomic DNA region around the target site compared with the reference wild-type sequence (Ref), HIE clone 1 contains a 49 bp frameshift mutation in both alleles of the target genes, indicating that the clone is a KO clone. b, HIE clone 2 contains no mutation in both alleles of the target genes, indicating that the clone is a wild-type clone.