Fig. 6 ∣. STAT1-KO HIE cells are more susceptible to GII.3 infection.

a, Western blots of cell lysates extracted from WT and STA1-KO detected the endogenous STAT1 protein by the protein-specific antibody (indicated by an asterisk) and confirmed protein loss in the isogenic KO culture. b, Gll.3 HuNoV-positive cells (green) were detected after staining with guinea pig anti-HuNoV Ab with nuclei (blue) stained by DAPI. Infected cells in clusters indicate viral spreading (40× objective lens). c, Numbers of infected cells were calculated in six independent images per condition, and each experiment was performed in triplicate (n = 18 in total). Error bars denote standard deviation of the data. Comparisons were made using the two-tailed Student's t-test. *P < 0.05. Figure reproduced with permission from ref. ¹².