| LentiCRISPRv2 (~100 ng/μL) | 20 μL (−2 μg) |
| BsmBI (NEB, cat. no. R0580S) | 1.5 μL |
| 10× NEB buffer 3.1 | 4 μL |
| ddH2O | 14.5 μL |
| Total volume | 40 μL |
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