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. Author manuscript; available in PMC: 2022 May 2.
Published in final edited form as: Nat Protoc. 2022 Feb 23;17(4):1004–1027. doi: 10.1038/s41596-021-00669-0
Gene Target site (5′-3′) sgRNA Fwd/Rev (5′-3′) Sequencing primers, Fwd/Rev (5′-3′)
STATl TCATGACCTCCT
GTCACAGC TGG
CACC G TCATGACCTCCTG TCACAGC ACCACATAAACTGCTGCTAAA
AAAC GCTGTGACAGGAGGTCAT GA C ACTGATACCTAAGAGGTGATACAT