Gene | Target site (5′-3′) | sgRNA Fwd/Rev (5′-3′) | Sequencing primers, Fwd/Rev (5′-3′) |
---|---|---|---|
STATl | TCATGACCTCCT GTCACAGC TGG |
CACC G TCATGACCTCCTG TCACAGC | ACCACATAAACTGCTGCTAAA |
AAAC GCTGTGACAGGAGGTCAT GA C | ACTGATACCTAAGAGGTGATACAT |
Gene | Target site (5′-3′) | sgRNA Fwd/Rev (5′-3′) | Sequencing primers, Fwd/Rev (5′-3′) |
---|---|---|---|
STATl | TCATGACCTCCT GTCACAGC TGG |
CACC G TCATGACCTCCTG TCACAGC | ACCACATAAACTGCTGCTAAA |
AAAC GCTGTGACAGGAGGTCAT GA C | ACTGATACCTAAGAGGTGATACAT |