| LentiCRISPRv2-BsmBI digested (Step 9) | 1 μL |
| Insert (1:400 diluted; Step 12) | 1 μL |
| T4 DNA ligation buffer | 0.5 μL |
| T4 DNA ligase | 0.5 μL |
| ddH2O | 2 μL |
| Total | 5 μL |
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