| Colony | 1× |
|---|---|
| 10 μM hU6-Fwd primer | 0.5 μL |
| 10 μM of sgRNA-Rev oligo (dilute from the 100 μM stock ordered in Step 5) | 0.5 μL |
| 2× Platinum Green Hot Start PCR Master Mix | 10 μL |
| ddH2O | 9 μL |
| Total | 20 μL |
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