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. Author manuscript; available in PMC: 2022 May 2.
Published in final edited form as: Nat Protoc. 2022 Feb 23;17(4):1004–1027. doi: 10.1038/s41596-021-00669-0
Reagent Amount 6× for six wells
PEI (1 μg/μL), diluted with ddH2O 7 μL 42 μL
DMEM without serum 160 μL 960 μL