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. Author manuscript; available in PMC: 2022 May 2.
Published in final edited form as: Nat Protoc. 2022 Feb 23;17(4):1004–1027. doi: 10.1038/s41596-021-00669-0
Reagent (plasmids prepared by midiprep in Step 26) Amount 6× for six wells
pMD2.G (~1 μg/μL) 0.6 μg 3.6 μL
psPAX2 (~1 μg/μL) 1 μg 6 μL
Lentiviral vector (~1 μg/μL) 1.6 μg 9.6 μL