| Reagent | Volume (total: 40 mL) |
|---|---|
| Filtered supernatant | 27 mL (adjust by adding more 1× DMEM) |
| 50% (wt/vol) PEG-6000, dissolved in ddH2O and autoclaved | 6.4 mL |
| 4 M NaCI | 2.9 mL |
| 1× PBS | 3.0 mL |
The PEG-6000, NaCI and PBS are all autoclaved or sterile.
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