Fig. 4. (a) Drug release profiles of AmpB-loaded HIPEC-3 aggregates at 35 °C, 36.5 °C and 38 °C in water. (b) Cytotoxicity of HIPEC-3 on normal hepatocytes (HL-7702) at temperatures below and above the LCST. (c, d) Confocal fluorescence images of HL-7702 cells incubated by PI and Hoechst 33342 at the same time. (e) Merged image of (c) and (d), and the corresponding bright-field image. (Blue color: Hoechst 33342 staining occurring in the cell nucleus, λex = 405 nm, λem = 421–501 nm; red color: PI background fluorescence occurring outside the cells, λex = 559 nm, λem = 577–657 nm).
