Fig. 3. Western blot analysis of proteins in mitochondrial signaling pathway in skeletal muscle tissues of mice. (a) Band images of relevant proteins: Sirt1, Sirt3, PGC-1α, NRF1, TFAM with GAPDH used as internal reference. (b) The relative expression of Sirt1, Sirt3, PGC-1α, NRF1 and TFAM. The expressions in WT mice are normalized to 1.0. Data are presented as mean ± SD (n = 6). *P < 0.05, **P < 0.01, ***P < 0.001 versus with ob/ob group.