The primer sequences.
| Gene | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
|---|---|---|
| Rat β-actin | CCCATCTATGAGGGTTACGC | TTTAATGTCACGCACGATTTC |
| Rat PGC-1α | CGCACAACTCAGCAAGTCCTC | CCTTGCTGGCCTCCAAAGTCTC |
| Rat Sirt1 | CAGCATCTTGCCTGATTTGTAA | TTGGAATTAGTGCTACTGGTCTTAC |
| Rat Sirt3 | TCTACAGCAACCTTCAGCAGTATG | AAGGAAGTAGTGAGCGACATTGG |
| Rat mtDNA | AGCTATTAATGGTTCGTTTGT | AGGAGGCTCCATTTCTCTTGT |
| Rat NRF1 | AGAGACAGCAGACACGGTTG | GCTGCGCCAAACACCTTAAA |
| Rat TFAM | AGCAAATGGCTGAAGTTGGG | TCTAGTAAAGCCCGGAAGGT |
| Mouse β-actin | GGCTGTATTCCCCTCCATCG | CCAGTTGGTAACAATGCCATGT |
| Mouse mtDNA | TAAATTTCGTGCCAGCCACC | GTTGACACGTTTTACGCCGA |
| Mouse PGC-1α | ATCTGACCACAAACGATGACCCT | GAGGCATCTTTGAAGTCTAGTTGTCTA |
| Mouse Sirt1 | GCTGACGACTTCGACGACG | TCGGTCAACAGGAGGTTGTCT |
| Mouse Sirt3 | TACAGGCCCAATGTCACTCA | GGATCCCAGATGCTCTCTCA |
| Mouse TFAM | ATTCCGAAGTGTTTTTCCAGCA | TCTGAAAGTTTTGCATCTGGGT |
| Mouse NRF1 | CGGAAACGGCCTCATGTGT | CGCGTCGTGTACTCATCCAA |