The relative expressions of mRNA including Sirt1, Sirt3, PGC-1α, NRF1 and TFAM in L6 cells. The expressions of mRNA in normal L6 cells are normalized to 1.0a.
| PGC-1α siRNA | − | + | + | + | + | + |
| FYGL (μg mL−1) | Normal | Control | 25 | 50 | 100 | 150 |
| Relative expression of Sirt1 mRNA | 1.0 ± 0.1** | 0.382 ± 0.003 | 0.46 ± 0.03 | 0.495 ± 0.005* | 0.81 ± 0.06* | 1.29 ± 0.09** |
| Sirt3 mRNA | 1.0 ± 0.2** | 0.23 ± 0.04 | 0.21 ± 0.05 | 0.56 ± 0.08* | 1.05 ± 0.08** | 1.4 ± 0.1*** |
| PGC-1α mRNA | 1.00 ± 0.05*** | 0.19 ± 0.02 | 1.8 ± 0.1*** | 6 ± 1*** | 15 ± 2*** | 22 ± 2*** |
| NRF1 mRNA | 1.0 ± 0.2* | 0.49 ± 0.07 | 1.8 ± 0.3*** | 3.02 ± 0.09*** | 5.5 ± 0.6*** | 21 ± 1*** |
| TFAM mRNA | 1.00 ± 0.07* | 0.56 ± 0.06 | 2.5 ± 0.3*** | 10 ± 1*** | 29 ± 1*** | 38 ± 1*** |
| mtDNA | 1.00 ± 0.06*** | 0.080 ± 0.005 | 0.29 ± 0.05 * | 1.3 ± 0.2*** | 2.3 ± 0.6*** | 2.1 ± 0.2*** |
a
Data are presented as mean ± SD (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 versus with control group.