Fig. 4. Effects of PHSP(hp) and GLSP(hp) on TNF-α and IL-6 production in RAW264.7 cell supernatant. RAW264.7 cells were incubated with PHSP(hp) and GLSP(hp) (0, 25, 50, 100, 200 μg mL−1) for 24 h and the cultural supernatants were collected for ELISA. (A and B) Effects of PHSP(hp) and GLSP(hp) on the secretion of TNF-α. (C and D) Effects of PHSP(hp) and GLSP(hp) on the secretion of IL-6. PBS served as a negative control and 2 μg mL−1 LPS served as a positive control. Data are present as mean ± SD (n = 3). *P < 0.05 versus the PBS group, **P < 0.01 versus the PBS group.
