(A and B) T-bet protein amounts in cNK cells and ILC1s from Tbx21+/+, Tbx215M/5M and Tbx21−/− mice were measured (A). Total cNK and ILC1 cell number in livers from Tbx21+/+ (n=13) and Tbx215M/5M (n=14) was calculated (B). Mean ± SD.
(C) Purified cNK cells and ILC1s from Tbx21+/+ (n=4) and Tbx215M/5M (n=3) mice were stimulated with IL-12 and IL-18 for 5 hours in the presence of monensin. The percentage of IFN-γ-producing cells was calculated. Mean ± SD.
Data are representative of two (A-C) independent experiments. See also Figure S4 and S5.