FIGURE 3.

Schematic representation of the construct used in the luciferase assays. A fragment of 760 bp of the ICOS 3′UTR, encompassing rs4404254 wild‐type allele (a) or rs4404254 allelic variant (c) the putative responsive elements for miR‐1276 or encompassing rs4675379 wild type (b) or rs4675379 allelic variant (d), the putative responsive elements for miR‐2355‐3p, was cloned in pGL3 vector downstream to the Firefly luciferase coding sequence. Nucleotides in miRNA sequence paired with ICOS 3′UTR are shown in capital letters and miRNA nucleotide paired with the corresponding allelic variant of ICOS 3′UTR SNPs is underlined in bold