FIGURE 4.

miR‐1276 and miR‐2355‐3p target the ICOS 3′UTR. 293T cells were transfected independently with pGL3‐Report luciferase plasmid or pICOS‐3′UTR vector together with either specific microRNA miR‐1276 or miR‐2355‐3p. Forty‐eight hours after transfection, the percentage of cells expressing the protein luciferase was measured by flow cytometry and normalized to the Firefly control. The results represent the mean and standard error (SE) of at least three independent experiments, each carried out in triplicate. The risk allele for rs4404254 SNP was (C) allelic variant (SNP1 mut) and the non‐risk allele was (T) allelic variant (SNP1 wt), while for rs4675379 SNP the risk allele for was (C) allelic variant (SNP2 mut), and the non‐risk allele was (G) allelic variant (SNP2 wt)