Detection and recovery of AA, DA and UA in real samples (n = 3)a.
| Sample no. | Species | Spiking (μM) | Detected (μM) | Recovery (%) | RSD (%) |
|---|---|---|---|---|---|
| Human serum1 | AA | 1200 | 1241.8 | 103.5 | 1.71 |
| DA | 120 | 119.5 | 99.6 | 3.38 | |
| UA | 120 | 121.5 | 101.2 | 1.20 | |
| Human serum2 | AA | 1800 | 1810.4 | 100.6 | 1.03 |
| DA | 180 | 183.0 | 101.7 | 1.89 | |
| UA | 180 | 178.3 | 99.0 | 2.21 | |
| Human urine1 | AA | 1200 | 1199.0 | 99.9 | 1.34 |
| DA | 120 | 121.6 | 101.3 | 2.30 | |
| UA | 120 | 124.3 | 103.6 | 2.45 | |
| Human urine2 | AA | 1800 | 1783.7 | 99.1 | 1.35 |
| DA | 180 | 180.1 | 100.0 | 0.76 | |
| UA | 180 | 173.2 | 96.2 | 3.12 |
a
The number “1” and “2” samples were prepared separately with different concentrations of AA, DA, and UA.