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. 2022 Apr 7;18(4):e1010217. doi: 10.1371/journal.ppat.1010217

Fig 1. ALCScV infection influences the gibberellin (GA) pathway in N. benthamiana plants.

Fig 1

(A) Photographs of the mock-, ALCScV- and ALCScV-mC4-inoculated N. benthamiana plants taken at 28 dpi. The results were reproduced in three independent experiments using 10 plants per treatment. (B) qRT-PCR analyses of the relative expression of GA pathway-associated genes in mock- or ALCScV-inoculated N. benthamiana plants. ** indicates a significant difference between the two treatments at the P < 0.01 level by Student’s t-test; * indicates a significant difference between the two treatments at the P < 0.05 level. These experiments were performed with three independent biological replicates with similar results. (C) and (D) Results of HPLC analyses showing the contents of endogenous GA in the PVX-, PVX-C4-, mock-, ALCScV-, and ALCScV-mC4-inoculated N. benthamiana plants at 14 dpi, respectively. Different letters above the bars indicate significant differences (P < 0.05).