Fig 7. Inhibition of NbGAI degradation is C4 concentration dependent.

(A) Agrobacterium culture carrying pGAI-GFP and Agrobacterium culture expressing C4-His (diluted to OD600 = 0, 0.25, 0.5 and 1.0, respectively) were co-infiltrated into N. benthamiana leaves. Two days later, total protein was extracted from different tissue samples and analyzed through Western blot analyses using an anti-GFP or an anti-His antibody. The CBB-stained RuBisCo large subunit gel shows equivalent sample loadings. (B) Western blot analysis shows that ALCScV C4 does not affect the stability of GFP in vivo.