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. 2022 Apr 7;18(4):e1010217. doi: 10.1371/journal.ppat.1010217

Fig 10. TbCSV C4 interacts with NbGAI in vitro and in vivo.

Fig 10

(A) The Y2H assay shows the interaction between TbCSV C4 and NbGAI in yeast cells. (B) The BiFC assay shows the interaction between TbCSV C4 and NbGAI in N. benthamiana leaf cells. The agroinfiltrated leaves were harvested at 48 hpi and examined under a confocal microscope equipped with a FITC filter. Bars = 100 μm. (C) N. benthamiana plants were inoculated with TRV-GUS and TbCSV or TRV-NbGAI and TbCSV. The TRV-GUS- and TbCSV-inoculated plants showed severe leaf curling symptoms, while the TRV-NbGAI and TbCSV-inoculated N. benthamiana plants showed mild leaf curling symptoms at 7 dpi. The results were reproduced in three independent experiments using 10 plants per treatment. (D) The qPCR analysis shows the accumulation level of TbCSV DNA. ** indicates a significant difference between the two treatments at the P < 0.01 level with Student’s t-test. These experiments were performed with three independent biological replicates with similar results.