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. 2022 Mar 23;119(13):e2117640119. doi: 10.1073/pnas.2117640119

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Copyright © 2022 the Author(s). Published by PNAS.

This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 4. — KCNQ2 and KCNQ5 form a complex in the brain. (A) Representative sequence coverage of KCNQ2 channels from anti-FLAG (M2 antibody) immunoprecipitated proteins followed by mass spectrometry analysis. Yellow indicates recovered peptides. (B) Total nonnormalized spectral counts of KCNQ2, KCNQ3, and KCNQ5 channels identified in Kcnq2^+/+;Kcnq3^+/+ (cortex, n = 7; hippocampus, n = 4), Kcnq2^flag/flagKcnq3^+/+ (cortex, n = 11; hippocampus, n = 8), and Kcnq2^flag/flag;Kcnq3^−/− (cortex, n = 5; hippocampus, n = 5). Data are displayed as box and whisker plots. (C) Volcano plot comparing calculated log₂ fold change using unnormalized spectral count values of different cortical proteins identified in Kcnq2^+/+;Kcnq3^+/+ (n = 7) and Kcnq2^flag/flag;Kcnq3^+/+ (n = 7) mice. (D) Immunoblot showing immunoprecipitated proteins from HEK293T cells expressing various 3XFLAG-KCNQ2, KCNQ3, and KCNQ5 channel combinations. Immunoprecipitation was performed using an anti-KCNQ5 antibody. n indicates number of mice.