Intra-day and inter-day (accuracy and precision) of established method^a.

HLM matrix	LLQC (5 ng mL−1)		LQC (15 ng mL−1)		MQC (150 ng mL−1)		HQC (400 ng mL−1)
	Intra-day assayb	Inter-day assayc	Intra-day assay	Inter-day assay	Intra-day assay	Inter-day assay	Intra-day assay	Inter-day assay
Mean	5.28	5.32	14.85	14.75	148.18	147.18	397.32	393.32
SD	0.08	0.05	0.23	0.38	2.49	2.71	2.76	6.70
Precision (%RSD)	1.52	0.99	1.52	2.58	1.68	1.84	0.70	1.70
% accuracy	−5.65	−6.36	1.00	1.69	1.21	1.88	0.67	1.67

^aHLM, human liver microsome; LLQC, lower limit quality control; LQC, low quality control; MQC, medium quality control; HQC, high quality control.

^bMean of twelve replicates on the same day.

^cMean of six replicates for three days.