Fig. 5. Evaluation of the cellular activity of compound DC_HG24-01. (a) The IC₅₀ curve of DC_HG24-01 against MV4-11 leukemia cell proliferation. (b) Cell proliferation inhibition assay of DC_HG24-01 on MRC5 and HUV-EC-C normal cells. (c) Western blot analysis of H3K14ac in MV4-11 leukemia cells treated with the 0 μM, 25 μM, 50 μM, 100 μM for 48 h. (d and e) Cell cycle analysis. MV4-11 cells were treated with 0 μM, 10 μM, 20 μM and 40 μM DC_HG24-01 for 24 h. (f and g) Cell apoptosis analysis. MV4-11 cells were treated with 0 μM, 10 μM, 20 μM and 40 μM DC_HG24-01 for 72 h. Data in panels (a and b) and (e–g) is represented as mean ± SD, n = 3.