Fig. 2. Interference and overexpression of PTEN in caerulein-induced AR42J cells after the caerulein-induced AR42J cells were transfected with fluorescent dye labeled scramble-shRNA, PTEN-shRNA, pCDH, and PTEN RNA, respectively. (A) Green fluorescence observed under the fluorescence microscope displayed that the PTEN-shRNA treated cells exhibited the strongest signal. The bar represents 200 μm. qRT-PCR assay (B) and western-blot experiments (C) further confirming the highest levels of PTEN in the AP cells could be significantly down-regulated by PTEN-shRNA. The assays mentioned here were repeatedly performed at least three times. ^^P < 0.01 versus scramble-shRNA; *P < 0.05 and **P < 0.01 versus pCDH.