Fig. 3. Evaluation of the effect on AP cell growth inhibition and the underlying mechanisms by MTT assay and cell cycle determination, respectively. (A) Results of the MTT assay showed that the AP-AR42J cells transfected with PTEN displayed the lowest cell viability, indicating that overexpression of PTEN could inhibit the proliferation of AP-AR42J cells. (B) Quantitative analysis of the ability of PTEN inhibited proliferation of AP-AR42J cells. (C) Cell cycles of AP-AR42J cells post transfected with various genes (including scramble-shRNA, PTEN-shRNA, pCDH, and PTEN RNA) confirmed that PTEN inhibited the growth of APAR42J cells mainly by blocking the G1 phase of cells. The experiments above were repeated three times. *P < 0.05 versus pCDH.