Fig. 2. The effects of miR-124 or miR-142 on the chemosensitivity of CDDP-resistant NSCLC cells. H1299/CDDP cells were transfected with miR-NC, miR-124, miR-nc, or miR-142. (A) The expressions of miR-124 and miR-142 in H1299 and H1299/CDDP cells were measured by qRT-PCR. (B–D) MTT and flow cytometry assays were performed to determine IC50 value, cell proliferation, and apoptosis in H1299/CDDP cells. (E and F) Western blot and FITC-LC3 punctate formation were carried out to analyze cell autophagy. *P < 0.05.
