Fig. 3. The interaction between miR-124, miR-142 and SIRT1 in CDDP-resistant NSCLC cells. (A) The predicted miR-124 or miR-142 binding sites in the 3′UTR of SIRT1. (B) After H1299/CDDP cells were cotransfected with miR-124, miR-142, or respective controls and luciferase reporter plasmids carrying the wild-type or mutated 3′UTR of SIRT1, luciferase reporter assay was performed to detect luciferase activity. (C) The enrichments of miR-124, miR-142, and SIRT1 were detected by RIP assay. (D) The protein level of SIRT1 in H1299/CDDP cells transfected with miR-124, miR-142, or respective control was evaluated by western blot. *P < 0.05.