Fig. 5. miR-124 and miR-142 inhibited autophagy and facilitated apoptosis in CDDP-resistant NSCLC cells by targeting SIRT1. H1299/CDDP cells were transfected with miR-124, miR-142, or matched controls or combined with SIRT1 or pcDNA. (A) The protein levels of SIRT1 in H1299/CDDP cells were detected by western blot assay. (B–D) MTT and flow cytometry assays were performed to measure the IC50 value, cell proliferation, and apoptosis. (E and F) Western blot and FITC-LC3 punctate formation were performed to assess cell autophagy. *P < 0.05.
