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. Author manuscript; available in PMC: 2022 Aug 3.
Published in final edited form as: Nat Neurosci. 2022 Feb 3;25(2):238–251. doi: 10.1038/s41593-021-01005-1

Extended Data Fig. 4. Pmp2+ Schwann cells preferentially ensheath Chat+ axons.

Extended Data Fig. 4

(a) Validation of ChAT-Cre-tdT expression specificity. Representative expression of PMP2 (yellow) and ChAT-Ab (white) in ChAT-Cre-tdT (red) sciatic nerves. The two panels on the right have shown almost complete overlap between the antibody (white) and tdT reporter (red) for ChAT (scale bar = 50um, n=3 biologically independent mice). (b) ChAT+ axons are mostly ensheathed by Pmp2+ SCs. Representative imaging showing the expression of MBP (blue) and PMP2 (green) in ChAT-tdT (red) sciatic nerve. (scale bar = 50um, n=3 biologically independent mice). (c) Quantification of percentage of ChAT axons ensheathed by a Pmp2+ SCs in three different nerve types. 2-way anova with multiple comparisons. Sciatic vs Sural: P=0.0159, Sciatic vs Femoral: P= 0.0456, Sural vs Femoral: P=0.0009. Data are mean +/− SD (n=3 biologically independent mice).