LC-MS/MS optimized parameters.

LC			MS/MS
LC	Agilent 1200		MS	Agilent 6410 QqQ
Gradient mobile phase	A: 10 mM ammonium formate in H2O		ESI	Positive ESI
	B: acetonitrile			Drying nitrogen gas, 12 L min−1 flow rate, 60 psi pressure
	0.5 mL min−1 flow rate
	65 min run time
C18 column (Agilent eclipse plus)	250 mm length			350 °C
	4.6 mm ID			4000 V capillary voltage
	5 μm particle size		Collision gas	High purity nitrogen gas
	23 ± 2 °C		Modes	MS scan and PI
Gradient mobile phase	Time	% acetonitrile	Drug	CNB and its related metabolites
	0	5
	5	5
	40	60
	60	90
	65	5
			Analyzer	145 eV fragmentor voltage
				25 eV collision energy