Table 2.
Primers used for quantitative real-time PCR (qRT-PCR).
| Gene | Sequence (5’-3’) | Product length (bp) | Annealing temperature (°C) | Accession number |
|---|---|---|---|---|
| Bcl- 2 | F: ATCGTCGCCTTCTTCGAGTT R: ATCCCATCCTCCGTTGTCCT |
150 | 59 | Z11961.1 |
| Bax | F: GTGATGGCATGGGACATAGCTC R: TGGCGTAGACCTTGCGGATAA |
90 | 58 | XM_422067.4 |
| IL-10 | F: GGAGAGAGCGGAGGTTTCG R: TCCCGTTCTCATCCATCTGC |
118 | 59.86 | XM_025143715.1 |
| INF-γ | F: GCTCCCGATGAACGACTTGA R: TGTAAGATGCTGAAGAGTTCATTCG |
150 | 59 | NM_205149 |
| IL-2 | F: GCTTATGGAGCATCTCTATCATCA R: ACTCCTGGGTCTCAGTTGGT |
130 | 58 | NM_204153.1 |
| IL-1β | F: GGAGAGAGCGGAGGTTTCG R: TCCCGTTCTCATCCATCTGC |
118 | 59.86 | XM_025143715.1 |
| AR | F: CTACGTCGTCAAGTGGGCAA R: TTTGTGCATCCGGTACTCGT |
193 | 60.32 | NM_001040090. |
| Pgk2 | F: GTGCTAATCCTGCAAACGGC R: CGATGGGCAGTTCCAAAAGC |
190 | 60.18 | NM_204985.3 |
| Cyclin A1 | F: GAA ATA CAG GCC CAA GCC CT R: TCA CCG CCA AGT ACA GTG TC |
137 | 60 | XM 015278427.2 |
| Cyclin A2 | F: TAT GCT GCT CGC ATC GAA GT R: GGA ACT GGT TGA TCG TCG GA |
164 | 58 | NM 205244.2 |
| GAPDH | F: TCCTCCACCTTTGATGCG R: GTGCCTGGCTCACTCCTT |
144 | 60 | NM_204305.1 |