Fig. 2. VH3-48/VK1-12 lineage antibodies broadly bind and neutralize multiple influenza subtypes.
a Binding affinity (KD) of each mAb to HA proteins from a panel of group 1 and group 2 influenza A isolates as measured by BLI. Dashed line indicates 50 nM. b In vitro neutralizing activity of each mAb against multiple strains of H1, H3, H4, and H7 subtype IAVs, shown as IC50 values. MEDI8852 and 39.29 were included as positive controls while 8D6 was the negative control. Dashed line indicates 50 μg/ml. The hosts of non-human infecting IAV strains have been noted in the full name of IAV strains, while the other IAV strains without host annotation are human infecting. c ELISA binding EC50 values of each mAb to denatured or untreated HAs of H3N2 and H1N1. Representative data are shown from two independent experiments.