Fig. 5. N49HA2 is critical for the recognition of H3 epitope features by VH3-48/VK1-12 lineage antibodies.
a Sequence alignment of the conserved epitope 36-57HA2 of H1 and H3. b Dose-dependent binding of 28-12 to divergent mutants of H3 peptide 36-57HA2 compared with the wild-type peptide. c Sequence alignment of 36-57HA2 within multiple subtypes. H3/H4/H14, but not other subtypes have residue N in position 49HA2. d–f Dose-dependent reactivity of the VH3-48/VK1-12 lineage antibodies to peptide 36-57HA2 of multiple subtypes. 8D6 was included as the isotype control. Data are presented as mean values of duplicates (b, d, e, f). Representative data are shown from two independent experiments (b, d, e, f).