Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 2;13:2370. doi: 10.1038/s41467-022-30120-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 7 — a Heatmap of 599 genes that are differentially expressed in diaphragm muscles of mice bearing non-cachectic melanomas and either mock-treated (n = 9) or treated with rFibcd1 (n = 10), and mice that carry a cachectic melanoma and that are either mock-treated (n = 7) or treated with rFibcd1 (n = 6). rFibcd1 represses some of the gene expression changes that characterize diaphragms from mice implanted with cachectic versus the non-cachectic melanomas. The heatmaps are based on z-scores of group averages from baseline-adjusted log2(TMP) for genes with at least one significant call among the related comparison sets. b GO term analysis of genes that are significantly regulated (34 upregulated, 32 downregulated; P < 0.05 and 50% change; Supplementary Data 5) in the diaphragm of mice with cachectic melanomas, treated with rFibcd1 versus mock. c Expression of genes related to myofiber atrophy from dataset in b and Supplementary Data 5. Data are means ± SD. P values were determined as reported in the RNA-seq methods and refer to cachectic versus non-cachectic, and to cachectic+rFibcd1 versus cachectic; *P < 0.05, **P < 0.01, ***P < 0.001. The N refers to diaphragm muscles and it is indicated in the legend of a. d Analysis of motor function in mice with either cachectic or non-cachectic orthotopic melanoma xenografts, and which received 3 i.p. injections of either rFibcd1 or mock (BSA) a week before testing. Melanoma-induced cachexia significantly reduces motor function compared to mice implanted with non-cachectic cancer cells. Comparison of the treadmill capacity of rFibcd1- versus mock-treated cachectic mice shows a trend towards improved treadmill performance in response to rFibcd1 injection but this difference does not reach statistical significance. However, cachectic mice treated with rFibcd1 have a treadmill capacity that is also not statistically different from that of non-cachectic mice. Data are means ± SD, with mice bearing non-cachectic melanomas and either mock-treated (n = 7) or treated with rFibcd1 (n = 7), and mice bearing cachectic melanomas and either mock-treated (n = 8) or treated with rFibcd1 (n = 8). P values were determined by one-way ANOVA with Tukey’s post hoc test; *P < 0.05 and **P < 0.01. Source data are provided in the Source Data file.